2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER For Topiroxostat CAS 94413-64-6

2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER is an intermediate of Topiroxostat CAS number is 94413-64-6. Isonicotinic acid Methyl ester and methyl isonicotinic acid N-oxide is major impurities during the synthesis, because it is similar to the main impurity Isonicotinic acid Methyl ester, there is a certain difficulty in the content detection. A high-performance liquid chromatography purity method for the complete separation of 2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER CAS number is 94413-64-6 with adjacent impurity peaks by isocratic elution and good peak shape was invented. The following steps are as below:
1. Preparing a test solution: taking 2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER and dissolving it in a mobile phase or methanol to prepare a solution having a concentration of 0.5 mg/ml to 1.2 mg/ml as a test solution;
2. Preparation of impurity reference solution: taking methyl isonicotinic acid and methyl isonicotinic acid N-oxide, and dissolving in mobile phase or methanol to prepare a solution having a concentration of 0.5 mg/ml to 1.2 mg/ml as an impurity. Reference solution
3. High-performance liquid chromatography is used to detect the test solution and the impurity reference solution respectively. The detection conditions are as follows: the C18 column is used, and the stationary phase of the column is silica gel coated with ODS-3 on the surface; the mobile phase is methanol: buffer salt, The volume ratio is10%: 90%: 10%, injection volume: 20ul, flow rate of 0.5ml / min ~ 1.5ml / min; column temperature of 10 ℃ ~ 40 ℃, The detection wavelength is 200 nm to 300 nm;
4. Calculate the content of methyl isonicotinic acid and methyl isonicotinic acid N-oxide according to the peak area normalization method.
This method enables accurate analysis of the content of 2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER CAS number 94413-64-6.

Thera. Category: Anti-gout and hyperuricemia

Cas No.: 94413-64-6

Synonyms:2-CYANO-4-PYRIDINE CARBOXYLIC ACID METHYL ESTER;Methyl 2-cyanoisonicotinate;Methyl 2-cyanopyridine-4-carboxylate;Methyl 2-cyanois;4-Pyridinecarboxylicacid, 2-cyano-, Methyl ester;Topiroxostat impurity L;2-Cyano-4-carbomethoxypyridine;Topiroxostat Impurity 15ChemicalBook

NW: 162.15

M.W.: C8H6N2O2

Assay: ≥99%

Packing:Export worthy packing

Material Safety Data Sheet:Available on request